MCM10 Knockout cell line(KYSE-150)

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MCM10 Knockout cell line(KYSE-150) Order

MCM10 Knockout cell line(KYSE-150)
Catalog#
YKO-HJ090
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MCM10 Knockout cell line(KYSE-150)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
MCM10 Knockout cell line (KYSE-150)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
MCM10
Gene ID
55388
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Accession
CVCL_E0WU(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 3 (based on transcript MCM10-202)
Validation: PCR+Sanger Sequencing
MCM10 Knockout cell line(KYSE-150) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:KYSE-150		 STR Info (Cell bank)
Cell Line:KYSE-150
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12131213
D13S317 811811
D7S820 10111011
D16S539 911911
VWA 16171617
TH01 7979
AMEL XXXX
TPOX 8888
CSF1PO 12131213
D12S391 1922
FGA 2124
D2S1338 2525
D21S11 3031
D18S51 1414
D8S1179 1015
D3S1358 1516
D6S1043 1820
PENTAE 1218
D19S433 1515
PENTAD 1010
D1S1656 1517.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MCM10 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the KYSE-150 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MCM10 gene knockout MCM10 Knockout cell line (KYSE-150) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
MCM10 Knockout cell line(KYSE-150)
MCM10 Knockout cell line(KYSE-150)
Catalog#
YKO-HJ090
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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